SEMESTER - III
3.1 COMPUTER APPLICATIONS - I

RATIONALE

Information technology and computers have great influence on all aspects of our life. All our work places and the living environment are being computerized. In order to prepare the diploma holders to work in these environments and contribute to them, it is essential that they are exposed to this area of work or study. This exposure will enable the students to enter the world with confidence, live in these environments in a harmonious way and contribute to the productivity.

DETAILED CONTENTS
THEORY

1. Introduction to Computers

(i) Block diagram of a computer & overview of its working.

(ii) Interconnections of various peripherals with computers.

(iii) Input/output & secondary storage devices.

(iv) Classification of programming languages.

(v) Classification of computers.

2. Familiarization with Operating System

(i) Introduction to computer operating system (DOS, Windows’95).

(ii) Introduction to DOS structure, system files, batch files & configuration files.

(iii) Booting the system from floppy & hard disk.

(iv) Brief introduction to DOS internal & external commands.

(v) Familarisation with windows structures, its use and application.

3. Preparation of documents through Word Processing

  • 3.0 Idea of text editors like microsoft word, write etc.

  • 3.1 Opening a document

  • 3.2 Preparing documents inserting diagrams & tables

  • 3.3 Editing document

  • 3.3.1 Character, word and Line Editing

  • 3.3.2 Margin Setting, Paragraph alignment

  • 3.3.3 Block operations

  • 3.3.4 Spell checker

  • 3.3.5 Saving a document

  • 3.4 Printing a document

4. Information Presentation for Decision Making using Spread Sheet:

(Excel/Lotus 1-2-3 )

  • 4.1 Applications of spread sheet

  • 4.2 Structure of spread sheet

  • 4.3 Preparing spread sheet for simple data and numeric operations

  • 4.4 Using formulae in spread sheet operations

  • 4.5 Making tables, sorting and querying

  • 4.6 Creation of graphs, Pie charts, bar charts.

  • 4.7 Printing reports

5 Computer Aided Drafting: (CAD)

  • 5.1 Making simple drawings using features of CAD and Conforming the drafting specifications

  • 5.2 Saving and retrieving drawings

  • 5.3 Dimensioning

  • 5.4 Lettering

  • 5.5 Plotting drawings

PRACTICALS
  1. Identification of various parts of PC.

  2. Demonstration of dis-assembly and assembly of PC and interconnection of Input and output devices of PC.

  3. Installation of DOS and simple exercises on TYPE, REN, DEL, CD, MD, TREE, COPY, BACKUP commands.

  4. Grouping commands with batch files.

  5. Disk diagnose, correction of partitioning.

  6. Installation of windows 95.

  7. Familiarization with start menu, taskbar icons, windows explorer.

  8. Control panel of setting.

  9. Getting hardware recognised.

  10. Installation of MS-Office.

  11. MS-Word - Basics of letter writing, templates, wizards, formatting documents.

  12. Creating Graphics, tables, mail merge, etc. using MS-WORD.

  13. Building a simple worksheet using MS-EXCEL.

  14. Formulas for calculations, sorting data, etc.

  15. Creating Lotus 1-2-3 sheet.

  16. Formulas, Ranges and Functions of Lotus 1-2-3.

  17. Simple graphics.

  18. Importing and exporting graphics through CAD.

  19. Drawing of geometrical figures.

3.2 CLINICAL MICROBIOLOGY-III
(Parasitology and Virology)

RATIONALE

The candidates undergoing training medical laboratory technology are made to learn the techniques of collection of samples, their processing and identification of various pathogens like bacteria, parasites, viruses using different techniques. In addition the candidates are given training in the use of standard safety measures while handling injective materials. The basic knowledge of different diseases caused by various micro-organisms is also imparted. The training is aimed at making the students competent to isolate and identify the causative micro-organisms.

DETAILED CONTENTS
THEORY
  1. Introduction to Medical Parasitology

  2. General characteristics of protozoa and helminths

  3. Collection, transport, processing and preservation of samples for routine parasitological investigations

  4. Morphology, life cycle and lab-diagnosis of Giardia and Entamoeba

  5. Morphology, Life cycle and lab-diagnosis of Roundworms and Hookworms

  6. Morphology, life cycle and lab-diagnosis of T. solium and T. saginata

  7. Morphology, life cycle and lab-diagnosis of Malarial parasite with special reference to P.vivax and P.falciparum

  8. Laboratory diagnosis of hydated cyst and cysti-cercosis

  9. Concentration techniques for demonstration of Ova(principles and applications)

  10. Introduction to medical virology

  11. Classification of viruses

  12. Classification of medically important viruses (Rabies, Polio, HIV, Influenza)

  13. Collection, transportation and storage of samples for viral diagnosis

  14. Processing of samples for viral diagnosis (Egg inoculation and tissue culture)

PRACTICALS

1. Routine stool examination for detection of intestinal parasites with concentration methods:

  •  Saline preparation

  •  Iodine preparation

  •  Floatation method

  •  Centrifugation method

  •  Formal ether method

  •  Zinc sulphate method

2. Identification of adult worms from models/slides

  •  Tapeworm

  •  Tapeworm segments

  •  Ascaris

  •  Hookworms

  •  Pinworms

3. Malarial parasite:

  •  Preparation of thin and thick smears

  •  Staining of smears

  •  Examination of smears for malarial parasites (P.Vivax and P.falciparum)

  •  Demonstration of various stages of life cycle of malarial parasites from stained slides

3.3 CLINICAL HAEMATOLOGY-III

RATIONALE

The training in this subject is imparted to enable the students to carry out routine clinical laboratory investigation (blood, urine etc). He/she should be able to provide technical help for selected sophisticated haematological techniques with adequate knowledge of various principles. The training in laboratory safety is also provided

DETAILED CONTENTS
THEORY

1. Erythrocyte sedimentation rate(ESR)

  • 1.1 Introduction

  • 1.2 Various methods of estimation

  • 1.3 Factors on which ESR and PCV depends

  • 1.4 Interpretation

2. Various colour indicies; their brief description

3. Absolute Eosionophil counting

  • 3.1 Introduction

  • 3.2 Various methods

  • 3.3 Clinical importance

4. Reticulocyte counting

  • 4.1 Introduction

  • 4.2 Various methods of counting

  • 4.3 Clinical importance

5. Red Cell Fragility Test

  • 5.1 Principle and setting up the test

  • 5.2 Clinical importance

6. Anaemias

  • 6.1 Definition and types of anaemia; factor causing anaemia

  • 6.2 Plasma haemoglobin and foetal haemoglobin estimation

  • 6.3 Laboratory diagnosis of haemolytic anaemia

7. Physiological variation in the normal values of tests (Hb, TLC, DLC, PCV/ESR, platelats etc)
PRACTICALS
  1. ESR estimations (wintrobe and westergren)

  2. PCV (Wintrobe and capillary)

  3. Absolute eosinophil counting

  4. Reticulocyte counting

  5. Red cell fragility test

  6. Plasma haemoglobin estimation

  7. Foetal haemoglobin test

  8. Examination of colour indicies

 

3.4 CLINICAL BIOCHEMISTRY-III

RATIONALE

The candidates are imparted basic training of theoratical and practical aspects in the field of clinical biochemistry. The candidates are made to learn the technique of collection of clinical samples and their processing alongwith recording of data. The student will also obtain the basic knowledge of chemistry and metabolism of various metabolites which are routinely estimated in different diseases so that a clear understanding of the different tests is obtained. The students are also given basic training in safety measures, quality control and automation.

DETAILED CONTENTS
THEORY

1. Serum Bilirubin

  • 1.1 Formation and excretion of bilirubin

  • 1.2 Metabolism of bile pigments

  • 1.3 Conjugated and unconjugated bilirubin

  • 1.4 Principles and procedures of serum bilirubin estimation

  • 1.5 Normal and abnormal values

  • 1.6 Clinical importance

2. Inorganic phosphorus

  • 2.1 Principles and procedures of estimation

  • 2.2 Normal and abnormal values

  • 2.3 Clinical importance

3. SGOT and SGPT

  • 3.1 Principles and procedures of estimation

  • 3.2 Normal and abnormal values

  • 3.3 Clinical importance

4. ALP and ACP (Alkaline and Acid Phosphatase)

  • 4.1 Principles and procedures of estimation

  • 4.2 Normal and abnormal values

  • 4.3 Clinical importance

5. Serum Amylase

  • 5.1 Principles and procedures of estimation

  • 5.2 Normal and abnormal values

  • 5.3 Clinical importance

6. Renal function tests (RFT)

  • 6.1 Functions of kidney

  • 6.2 Renal clearnace tests

  • 6.3 Clinical importance

7. Uric acid estimation

  • 7.1 Principles and procedures of estimation

  • 7.2 Normal and abnormal values

  • 7.3 Clinical importance

8. Creatinine estimation

  • 8.1 Principles and procedures of estimation

  • 8.2 Normal and abnormal values

  • 8.3 Clinical importance

9. Serum Calcium

  • 9.1 Principles and procedures of estimation

  • 9.2 Normal and abnormal values

  • 9.3 Clinical importance

PRACTICALS

1. Routine and enzyme method of:

  •  Serum bilirubin estimation

  •  Phosphorous estimation

  •  Renal clearnace tests

  •  Serum uric acid estimation

  •  Serum creatinine estimation

  •  Serum calcium estimation

  •  SGOT estimation

  •  SGPT estimation

  •  ALP estimation

  •  ACP estimation

3.5 HISTOPATHOLOGY-I

RATIONALE

The training is aimed at preparing the students for preparing tissue sections of various types (paraffin/frozen) and stain them. He/she will able to provide special stains for detailed information. He/she should be able to collect exfolatine cytology smears, contact smears and perform fine needle aspiration cytology examination under supervision and carry out routine and special staining procedures. He/she should be able to display specimens for museum and help in performing autopsy.

DETAILED CONTENTS
THEORY

1. Definitions, sources and types of histological specimen (Biopsy) kinds of histological preservations

2. Labelling, fixation, properties of fixing fluids classification and composition of fixing fluids

3. Paraffin embedding, dehydration, clearing, impregnation and casting

4. Cutting of Tissue Sections

Care and use of microtomes, microtome knives honing and stropping techniques, attachment of block to block holder. Trimming, section cutting, errors in sectioning and remedies, collection of sections to slide from tissue floatation bath

5. Principles and staining techniques of

a) Routine

  •  Haemotoxylin and Eosin

b) Special

  •  PAS - Reticulin

  •  PTAH - Iron

  •  Calcium - AFB

  •  FAT

PRACTICALS

  1. Receiving specimen, labelling and cataloguing

  2. Preparation of fixatives, fixing of specimen

  3. Dehydrating, making solution of various reagents,clearing, impregnation and casting

  4. Embedding and cutting of sections

  5. Preparation of stains

  6. Routine (H&E) and special staining

3.6 E.C.G

RATIONALE

A diploma holder of medical laboratory technology is supposed to have some knowledge and skill in performing ECG and interpret the results.

DETAILED CONTENTS
PRACTICALS
  1. Introduction and basic principle along with types of leads

  2. Normal electrocardiogram

  3. Value of Electrocardiogram in health and disease

  4. Method of Analysis of the electrocardiogram

  5. Care and maintenance of E.C.G. Machine

Note : 1) The relevant theory will be taught along with practical exercise in the E.C.G. lab.

          2) There will be no theory paper in this subject.