CLINICAL
BIOCHEMISTRY - I
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L T P
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3 - 4
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RATIONALE
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The students are imparted basic
training of theoretical and practical aspects in the field of clinical
biochemistry. The students are made to learn the technique of collection of
clinical samples and their processing along with recording of data. The student
will also obtain the basic knowledge of chemistry and metabolism of various
metabolites which are routinely estimated in different diseases so that a clear
understanding of the different tests is obtained. The students are also given
basic training in safety measures, quality control and automation
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DETAILED CONTENTS
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1.
Introduction to Biochemistry
(4
hrs)
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1.1
Definition
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1.2
Importance of biochemistry
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1.3
SI Units and their use
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1.4
Volumetric apparatus and their calibration
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2.
Cleaning and storage of laboratory, glass and plastic
ware (8 hrs)
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2.1
Cleaning and care of laboratory glass and plastic ware
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2.2
Different cleaning agents (soaps, detergents, chromic
acid)
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2.3
Methods of cleaning and storage
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3.
Important instruments; principle of working, handling
and care of (18 hrs)
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3.1
Balance (Analytical, electrical/electronic)
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3.2
Centrifuge
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3.3
Colorimeter
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3.4
Spectrophotometer
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3.5
Flamephotometer
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3.6
Glucometer
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4.
Blood chemistry (14 hrs)
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4.1 Composition of blood and its functions
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4.2 Various anticoagulants and their uses
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4.3 Separation of serum and plasma
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4.4 Different protein precipitation agents
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4.5 Preparation of protein free filtrate
(PFF)
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5.
Collection and preservation of clinical specimens
(12
hrs)
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- Blood
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Urine
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-
Stool
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-
Body Fluids
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LIST OF PRACTICALS
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1.
Cleaning of glass
ware
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2.
Standardization of glass ware
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3.
Handling and maintenance of Balance, Centrifuge,
Colorimeter and Flame Photometer
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4.
Preparation of various anticoagulants and specimen
collection bottle
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5.
Collection of blood by various methods
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6.
Separation of serum and plasma
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7.
Preparation of different protein precipitating agents,
PFF preparation
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RECOMMENDED BOOKS
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1.
A Procedure Manual for Routine Diagnostic Tests Vol.
I by KL Mukherjee; Tata McGraw Hill
Publishers, New Delhi
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2.
Biochemistry Estimations by F.J.Baker
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SUGGESTED DISTRIBUTION OF MARKS
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