HAEMATOLOGY - I
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L
T P
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3 -
4
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RATIONALE
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The training in haematology is
imparted to enable the students to know the principle of tests, methodology of
routine as well as advanced procedures being carried out in the laboratory by
using routine sample as well as sophisticated instruments. Stress is also given
in use of safety measures in the laboratory
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DETAILED CONTENTS
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Theory
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1.
Introduction to haematology
(06
hrs)
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1.1
Various apparatus used in haematology labs.
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1.2
Precautions to be taken while working in a lab.
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1.3
Various hazards
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1.4
Factors affecting test results
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1.5
Role of technician in infection control
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2.
Apparatus and Instruments
(08
hr)
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Parts,
functions, principles/maintenance and working of microscope, centrifuge, water
bath, shaker, pH meter, hot air oven
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3.
Sterilization (06
hrs)
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3.1 Methods of sterilization
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3.2 Washing, cleaning and sterilization of
glass and plastic ware
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3.3 General and volumetric apparatus cleaning
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4.
Haemopoeisis (09
hrs)
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4.1
Erythropoiesis, leucopoeisis, thrombopoeisis
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4.2
Definition, composition and functions of blood
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4.3
Normal values
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5.
Anticoagulants (05
hrs)
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Definition and
various types of anticoagulants alongwith their mode of action and their
preparation with merits and demerits of each
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6.
Collection and preservation of blood (06
hrs)
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6.1
Collection of blood; venous and capillary
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6.2
Various equipment used for collection of blood samples
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6.3
Safety measures at the time of sampling and collection
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6.4
Preservation and disposal of processed blood samples
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7.
Romanowsky stains (08
hrs)
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7.1
Theory and preparation
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7.2
Choice of slide and spreader and preparation of blood film
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7.3
Characteristics of good film preparation
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7.4
Staining procedure and principle
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7.5
Effects of pH on staining
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LIST
OF PRACTICALS
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1.
Demonstration of various parts of centrifuge; its functioning
and care
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2.
Demonstration of various parts of microscope its functioning
and care
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3.
Cleaning and drying of glass and plastic ware
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4.
Preparation of various anticoagulants
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5.
Collection of venous and capillary blood
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6.
Preparation of the stains and other reagents
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7.
Preparation of peripheral blood film (PBF)
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8.
To stain a peripheral blood Film by Romanowsky - stain
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RECOMMENDED
BOOKS
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1.
Medical Laboratory Technology Vol. 1 by KL Mukherjee; Tata
McGraw Hill Publishers, New Delhi
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2.
An Introduction to Medical Laboratory Technology by FJ Baker;
Butterworth Heinmann, Oxford
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3.
Medical Laboratory Manual for Tropical Countries by Monica
Cheesbrough; Cambridge University Press, UK
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4.
Textbook of Medical Laboratory Technology by Praful B Godkar;
Bhalani Publishing House, Mumbai
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5.
Practical Haematology by JV Decei; ELBS with Curchill Living
Stone; UK
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6.
Medical Laboratory Science Theory and Practical by J Ochei and
A Kolhatkar, Tata McGraw Hill Publishing Company Ltd., New Delhi 2000 Ed.
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7.
Medical Lab. Technology by Satish Gupte, JP Publishers
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SUGGESTED DISTRIBUTION OF MARKS
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