CLINICAL
BIOCHEMISTRY - II
L T P
3 -
4
RATIONALE
The students are imparted basic training of theoretical
and practical aspects in the field of clinical biochemistry. The students are
made to learn the technique of collection of clinical samples and their
processing along with recording of data. The student will also obtain the basic
knowledge of chemistry and metabolism of various metabolites which are
routinely estimated in different diseases so that a clear understanding of the
different tests is obtained. The students are also given basic training in
safety measures, quality control and automation
DETAILED CONTENTS
Theory
1.
Blood Sugar Estimation
and Glucose Tolerance Test (GTT) (12 hrs)
1.1
Principle and methods of estimation
1.2
Reference values
1.3
True and apparent sugar
1.4
Renal threshold
1.5
Importance and Performance of GTT
1.6
Clinical importance of blood sugar and GTT
2.
Blood urea (8
hrs)
2.1
Formation and excretion of urea
2.2
Principle and procedures of different methods of urea
estimation
2.3
Reference values
2.4
Clinical Importance
3.
Serum Creatnine (4
hrs)
3.1
Principle and procedure of estimation
3.2
Reference values
3.3
Clinical importance
4.
Serum proteins (8 hrs)
4.1
Definition
4.2
Different methods of estimation including principles and
procedures
4.3
Reference values
4.4
Clinical importance
5.
Electrolytes and trace elements (8 hrs)
5.1
Functions of electrolytes (Na+, K+
and Cl-).
Other
essential elements like P, Ca2+, Fe 2+. Metabolism of
these ions
5.2
Principles and procedures of estimation
5.3
Reference values
5.4
Clinical importance
6.
Uric Acid Estimation (4
hrs)
6.1
Principles and procedures of estimation
6.2
Reference values
6.3
Clinical Importance
7.
Quality
Assurance in Biochemistry (4 hrs)
LIST OF PRACTICALS
- Estimation of blood sugar
(Folin-Wu method, O-toluidine method and enzymatic method)
- Performance of GTT
- Serum urea estimation
- Serum creatnine estimation
- Serum uric acid estimation
- Plasma and serum protein estimation
- Estimation of electrolyte
levels of Na+, K+ and Cl- by flame photometer and kit method
- Preparation of all types of reagents
RECOMMENDED BOOKS
- A Procedure Manual for Routine Diagnostic Tests Vol.
I and III by KL Mukherjee; Tata McGraw Hill Publishers, New Delhi
- A Textbook of Medical Laboratory Technology by P
Godkar; Bhalani Publishing House, Mumbai
SUGGESTED
DISTRIBUTION OF MARKS
Topic No.
|
Time allotted (Hrs)
|
Marks Allotted (%)
|
1
|
12
|
20
|
2
|
8
|
14
|
3
|
4
|
10
|
4
|
8
|
16
|
5
|
8
|
16
|
6
|
4
|
12
|
7
|
4
|
12
|
Total
|
48
|
100
|
|