CLINICAL  BIOCHEMISTRY - II

L   T    P  

3   -    4  

RATIONALE

 

The students are imparted basic training of theoretical and practical aspects in the field of clinical biochemistry. The students are made to learn the technique of collection of clinical samples and their processing along with recording of data. The student will also obtain the basic knowledge of chemistry and metabolism of various metabolites which are routinely estimated in different diseases so that a clear understanding of the different tests is obtained. The students are also given basic training in safety measures, quality control and automation

DETAILED CONTENTS

Theory

 

1.  Blood  Sugar Estimation and Glucose Tolerance Test (GTT)                                    (12 hrs)

 

1.1              Principle and methods of estimation

1.2              Reference values

1.3              True and apparent sugar

1.4              Renal threshold

1.5              Importance and Performance of GTT

1.6              Clinical importance of blood sugar and  GTT

 

2.  Blood urea                                                                                                       (8 hrs)

 

2.1              Formation and excretion of urea

2.2              Principle and procedures of different methods of urea estimation

2.3              Reference values

2.4              Clinical Importance

 

3.  Serum Creatnine                                                                                               (4 hrs)

 

3.1              Principle and procedure of estimation

3.2              Reference values

3.3              Clinical importance

 

4.  Serum proteins                                                                                                  (8 hrs)

 

4.1              Definition

4.2              Different methods of estimation including principles and procedures

4.3              Reference values

4.4              Clinical importance

 

5.  Electrolytes and trace elements                                                                          (8 hrs)

 

5.1              Functions of electrolytes (Na+, K+ and  Cl-). 

Other essential elements like P, Ca2+, Fe 2+. Metabolism of these ions

5.2              Principles and procedures of estimation

5.3              Reference values

5.4              Clinical importance

 

6.  Uric Acid Estimation                                                                                         (4 hrs)

 

6.1              Principles and procedures of estimation

6.2              Reference values

6.3              Clinical Importance

 

7.  Quality Assurance in Biochemistry                                                                      (4 hrs)

 

 

LIST OF PRACTICALS

 

  1. Estimation of blood sugar (Folin-Wu method, O-toluidine method and enzymatic method)

 

  1. Performance of GTT

 

  1. Serum urea estimation

 

  1. Serum creatnine estimation

 

  1. Serum uric acid estimation

 

  1. Plasma and serum protein estimation

 

  1. Estimation of electrolyte levels of Na+,  K+  and  Cl-  by flame photometer and kit method

 

  1. Preparation of all types of reagents

 

 

RECOMMENDED BOOKS

 

  1. A Procedure Manual for Routine Diagnostic Tests Vol. I and III by KL Mukherjee; Tata McGraw Hill Publishers, New Delhi

 

  1. A Textbook of Medical Laboratory Technology by P Godkar; Bhalani Publishing House, Mumbai

 

 

SUGGESTED DISTRIBUTION OF MARKS

 

Topic No.

Time allotted  (Hrs)

Marks Allotted (%)

1

12

20

2

8

14

3

4

10

4

8

16

5

8

16

6

4

12

7

4

12

Total

48

100