CLINICAL MICROBIOLOGY - II

(Bacteriology)

L    T     P 

3    -    4 

RATIONALE

 

The students undergoing training of medical laboratory technology learn the knowledge of basic morphology, staining, culture, biochemical characteristics and lab-diagnosis of pathogenic bacteria. In addition to this, they are also made aware about the examination of bacteria present in milk and water.

 

DETAILED CONTENTS

Theory

 

1.  Bacteriology                                                                                                     (20 hrs)

 

General Characteristics of Bacteria - Morphology, Staining, Culture, Biochemical caracters and Lab diagnosis of:                                                                      

 

1.1    Staphylococi                                                    

1.2    Streptococci and pneumococci

1.3    Corynebacterium diptheria

1.4    Enterobacteriacae - I (E coli, Klebsiella)

1.5    Enterobacteriacae – II (Salmonella, Shigella, Proteus)

1.6    Pseudomonas

1.7    Vibrio Cholerae

1.8    Neisseria

1.9    Clostridium

1.10      Treponema Pallidium

1.11      Mycobacterium tuberculosis

 

2.  Bacteriological examination of:                                                                             (8 hrs)

 

2.1              Water

2.2              Milk

 

3.  Bacterial Pathogenicity                                                                                       (4hrs)

 

3.1              Definition of pathogenicity, pathogenesis and virulence

3.2              Sources of infection

3.3              Mode of spread of infection

3.4              Types of infection
 

4.  Nosocomial Infection                                                                                           (6 hrs)

 

4.1              Introduction

4.2              Common types and source of nosocomial infection

4.3              Control of nosocomial infections

 

5.  Laboratory diagnosis of infectious diseases                                                              (10 hrs)

 

5.1              Respiratory tract infections

5.2              Wound and skin infections

5.3              Urinary tract infections

5.4              Genital tract infection

5.5              Gastro intestinal infections

5.6              PUO

 

LIST OF PRACTICALS

 

1.    Collection, transportation of clinical samples, processing including culture of following clinical samples for identification of pathogens – Urine, Stool, Sputum, Throat swabs, Pus and Pus swabs, Blood, Skin, Eye and Ear swabs and CSF

 

2.    Identification of pure bacterial cultures of common pathogens.

 

3.    Bacteriological examination of water and milk samples

 

INSTRUCTIONAL STRATEGY

 

The teacher should lay stress on general characteristics of bacteria, morphological features, nomenclature of bacterial for common use. The students should be made familiar with common names of bacteria and stress on correct use of bacterial pronunciation and spellings. The students should be taught with illustrations/audio-visual aids.

 

RECOMMENDED BOOKS

 

1.       Medical Laboratory Technology by Kanai Lal Mukherjee; Tata McGraw Hill, New Delhi

 

2.       An Introduction to Medical Laboratory Technology by FJ Baker; Butterworth – Heinemann; Oxford

 

3.       Text Book of Microbiology by Ananthanarayan and Paniker; Orient Longman, Hyderabad

 

4.       Textbook of Medical Microbiology by Satish Gupta; JP Brothers, New Delhi

 

5.       Practical Book of Medical Microbiology by Satish Gupta; JP Brothers, New Delhi

 

6.       Medical Laboratory Manual for Tropical Countries Vol. I and II by Monica Cheesbrough; Cambridge University Press; UK

 

7.       Textbook of Medical Laboratory Technology by Praful B Godkar; Bhalani Publishing House, Mumbai

 

8.       Text book of Medical Microbiology by Cruckshiank Vol. I

 

9.       Textbook of Medical Microbiology by Greenwood, ELBS

 

10.      Medical Laboratory Science by Jockie and Kolhatkar, Tata McGraw Hill.

 

SUGGESTED DISTRIBUTION OF MARKS

 

Topic No.

Time allotted  (Hrs)

Marks Allotted (%)

1

20

40

2

8

15

3

4

10

4

6

15

5

10

20

Total

48

100