HAEMATOLOGY - II
L T P
3 - 4
RATIONALE
The training in haematology is
imparted to enable the students to know the principle of tests, methodology of
routine as well as advanced procedures being carried out in the laboratory by
using routine as well as sophisticated instruments. Stress is also given in use
of safety measures in the laboratory
DETAILED CONTENTS
Theory
1.
Differential leucocyte counting (DLC) (06 hrs)
1.1 Preparation and staining of blood film
1.2 Performance of DLC
1.3 Normal values and significance of DLC
1.4 Blood cell morphology in health and disease
2.
Haemoglobinometry (12
hrs)
2.1 Formation of haemoglobin, function and its degradation
2.2 Types of haemoglobin
2.3 Various methods of estimation with specific reference to
cyanmethaemoglobin method
3.
Haemocytometery (18
hrs)
3.1 Various counting chambers
3.2 Methods of counting of RBC, WBC and platelets, their
calculation and reference values.
3.3 Errors involved in haemocytometery and means to minimize them
4.
Quality Assurance in haematology (06
hrs)
5.
Automation in haematology (06
hrs)
LIST OF PRACTICALS
1. Preparation of peripheral blood film and recognition of
different cellular components
2. Preparation and standardization of stains (leishman and
giemsa)
3. Preparation of thick and thin blood smear
4. Haemoglobin Estimation by Oxy-Hb and Cyanmethaemoglobin method
5. Counting of RBC
6. Counting of WBC
7. Platelet counting
8. Absolute eosinophil counting
9. Study of morphology of normal RBC and WBC with the help of
stained slide
10. To study abnormal morphology of RBC with the help of stained
slide
11. To study abnormal morphology of WBC with the help of stained
slide
12. To study abnormal morphology of platelet with the help of
stained slide
RECOMMENDED BOOKS
- Medical Laboratory Technology Vol. 1 by KL Mukherjee;
Tata McGraw Hill Publishers, New Delhi
- An Introduction to Medical Laboratory Technology by
FJ Baker; Butterworth Heinmann, Oxford
- Medical Laboratory Manual for Tropical Countries by
Monica Cheesbrough; Cambridge University Press, UK
- Textbook of Medical Laboratory Technology by Praful B
Godkar; Bhalani Publishing House, Mumbai
- Practical Haematology by JV Decei; ELBS with Curchill
Living Stone; UK
- Medical Laboratory Science Theory and Practical by J
Ochei and A Kolhatkar, Tata McGraw Hill Publishing Company Ltd., New Delhi
2000 Ed.
SUGGESTED DISTRIBUTION OF MARKS
Topic No.
|
Time allotted (Hrs)
|
Marks Allotted (%)
|
1
|
6
|
12
|
2
|
12
|
24
|
3
|
18
|
40
|
4
|
6
|
12
|
5
|
6
|
12
|
Total
|
48
|
100
|