HAEMATOLOGY - II

L   T   P  

3   -   4 

RATIONALE

 

The training in haematology is imparted to enable the students to know the principle of tests, methodology of routine as well as advanced procedures being carried out in the laboratory by using routine as well as sophisticated instruments. Stress is also given in use of safety measures in the laboratory

 

DETAILED CONTENTS

Theory

 

1. Differential leucocyte counting (DLC) (06 hrs)

1.1     Preparation and staining of blood film

1.2     Performance of DLC

1.3     Normal values and significance of DLC

1.4     Blood cell morphology in health and disease

 

2. Haemoglobinometry (12 hrs)                                                                      

2.1     Formation of haemoglobin, function and its degradation

2.2     Types of haemoglobin

2.3     Various methods of estimation with specific reference to cyanmethaemoglobin method

 

3. Haemocytometery (18 hrs)                                                                                              

3.1     Various counting chambers

3.2     Methods of counting of RBC, WBC and platelets, their calculation and reference values.

3.3     Errors involved in haemocytometery and means to minimize them

 

4.  Quality Assurance in haematology  (06 hrs)                                                          

5.  Automation in haematology  (06 hrs)                                                           

 

LIST OF PRACTICALS

1.     Preparation of peripheral blood film and recognition of different cellular components

2.     Preparation and standardization of stains (leishman and giemsa)

3.     Preparation of thick and thin blood smear

4.     Haemoglobin Estimation by Oxy-Hb and Cyanmethaemoglobin method

5.     Counting of RBC

6.     Counting of WBC

7.     Platelet counting

8.     Absolute eosinophil counting

9.     Study of morphology of normal RBC and WBC with the help of stained slide

10.    To study abnormal morphology of RBC with the help of stained slide

11.    To study abnormal morphology of WBC with the help of stained slide

12.    To study abnormal morphology of platelet with the help of stained slide

 

RECOMMENDED BOOKS

  1. Medical Laboratory Technology Vol. 1 by KL Mukherjee; Tata McGraw Hill Publishers, New Delhi
  1. An Introduction to Medical Laboratory Technology by FJ Baker; Butterworth Heinmann, Oxford
  1. Medical Laboratory Manual for Tropical Countries by Monica Cheesbrough; Cambridge University Press, UK
  1. Textbook of Medical Laboratory Technology by Praful B Godkar; Bhalani Publishing House, Mumbai
  1. Practical Haematology by JV Decei; ELBS with Curchill Living Stone; UK
  1. Medical Laboratory Science Theory and Practical by J Ochei and A Kolhatkar, Tata McGraw Hill Publishing Company Ltd., New Delhi 2000 Ed.

 

SUGGESTED DISTRIBUTION OF MARKS

 

Topic No.

Time allotted  (Hrs)

Marks Allotted (%)

1

6

12

2

12

24

3

18

40

4

6

12

5

6

12

Total

48

100